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BSC 108 Lab 4 Enzyme-Controlled Reactions Lab and Journal Worksheet (Alabama)

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BSC 108 Lab 4 Enzyme-Controlled Reactions Lab and Journal Worksheet (Alabama)

Experiment 2 - The Effect of pH on Amylase Enzyme Activity

Experiment 3 - The Effect of Temperature on Amylase Enzyme Activity

Instructions: Answer the questions below, based on Experiments 2 and 3.

1. What are the enzyme, substrate, and product in this experiment?

2. Formulate a hypothesis. What do you believe will be the optimum pH for this enzymatic reaction?

3. At which pH or pH range does amylase enzyme function best?

4. In which ranges of pH will amylase cease to function?

5. Formulate a hypothesis. What do you believe will be the optimum temperature for this enzymatic reaction?

6. What temperature is optimum for this enzyme?

7. Why does the enzyme only function in a limited range of temperature?

8. Do the optimal conditions for amylase function correspond with the usual conditions of the human body?

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